Translational_Unit

Part:BBa_K1641007:Design

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-09)


Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Flexible chain works to keep two parts away to avoid misfolding; ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Scre has similar structure of Cre, and hence have far better performance if EGFP is fused to its C-term rather than N-term.


Source

Scre from BBa_K1641001 and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks.

References